In Rio de Janeiro, baby macaw, Blu, is captured by dealers and smuggled to the USA. While driving through Moose Lake, Minnesota, the truck that is transporting Blu accidentally drops Blu's box on the road. A girl, Linda, finds the bird and raises him with love. Fifteen years later, Blu is a domesticated and intelligent bird that does not fly and lives a comfortable life with bookshop owner Linda. Out of the blue, clumsy Brazilian ornithologist, Tulio, visits Linda and explains that Blu is the last male of his species, and he has a female called Jewel in Rio de Janeiro. He invites Linda to bring Blu to Rio so that he and Jewel can save their species.
It's a jungle out there for Blu, Jewel and their three kids after they're hurtled from Rio de Janeiro to the wilds of the Amazon. As Blu tries to fit in, he goes beak-to-beak with the vengeful Nigel, and meets the most fearsome adversary of all: his father-in-law.